Pediatric Cardiology and Cardiac Surgery
Vol.24 No.1 2008 (35-43)
Yoshimitsu Tsuzuki,1) Yuko Takeba,1), Toshio Kumai,1),Naoki Matsumoto,1) Kentaro Asoh,2) Kohtaro Murano,2),Masanori Mizuno,2) and Shinichi Kobayashi1)
Departments of 1)Pharmacology and 2)Pediatrics, St. Marianna University School of Medicine, Kanagawa, Japan
Background: We investigate the effects of antenatal glucocorticoid (GC) on the fetal and neonatal expression of a variety of proteins related to cardiac function.
Methods: Dexamethasone (DEX) was administered to pregnant Wistar rats and fetuses of 19 and 21 days gestation, and 1-3- and 5-day-old neonatal rats were sacrificed. We identified the differentiated proteins from the fetal and neonatal hearts using a liquid chromatography mass spectrometry/mass spectrometry (LC/MS/MS) technique.
Results: In vehicle control groups, troponin T expression decreased as the maturational age of the fetuses and neonates increased. Tropinin T expression was increased significantly in the DEX group compared to vehicle controls in the 19- and 21-day fetuses. Furthermore, accompanying the increased troponin T expression, DEX increased the expression of cardiac-specific transcription factor GATA-4 in fetuses.
Conclusions: These results suggest that antenatal GC therapy increases expression of troponin T and may contribute to development on immature fetal myocardium.