【Introduction】Left ventricular (LV) apical rotation is an important component of cardiac function; however, the effects of load and inotropic state are still controversial. 【Methods】We measured peak LV apical rotation (Prot) and time to Prot from onset Q wave (Trot) by Doppler speckle tracking in global, subendcardial and subepicardial layers of the LV wall in 8 pigs with an open chest during control conditions, increased and decreased LV preload, increased LV afterload, and increased and decreased LV contractility. We recorded simultaneously ECG, LV pressure and volume. 【Results】Compared with control values, increased preload had strongly significant correlation on Trot in global and subendcardial layers (r = 0.91). The other side, decreased preload had significant correlation on Prot in global layer (r = 0.77), but delayed timing. Increased afterload had strongly significant correlation on Prot in all layers (r = 0.97), and Trot in global and subendcardial layers (r = 0.94). Increased contractility had no correlation on Prot and Trot. The other side, decreased contractility had significant correlation on Trot in all layers (r = 0.92 to 0.96). There is strongly significant difference between Prot in subendcardial and subepicardial layers of LV for all physiological runs (p < 0.001) with higher number at the subendmyocardium. 【Conclusion】Increased afterload does not affect peak and timing of the LV apical rotation. Decreased preload does not affect peak of the LV apical rotation, but delayed timing. Inner and outer layers of the LV wall have different rotational behaviors.